Two-Dimensional and Three-Dimensional Cell Culture and Their Applications

Cell culture is one of the most important and commonly used in vitro tools to comprehend various aspects cells or tissues a living body such as cell biology, tissue morphology, mechanism diseases, signaling, drug action, cancer research also finds its great importance preclinical trials drugs. There are two major types cultures that used- two-dimensional (2D) three-dimensional (3D). The former has been since 1900s, owing simplicity low-cost maintenance it forms monolayer, while latter being advanced version currently worked upon. This chapter intends provide true meaning significance both cultures. It starts by making clear distinction between proceeds further discuss their different applications vitro. 2D projected through assays therapeutic treatment understand motility whereas 3D includes models spheroid structures consisting multiple layers cells, puts light on use discovery development. concluded with detailed account production proteins cells.